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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MA1-074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAP1 Monoclonal Antibody (RL13)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-074 detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat, human, and mouse samples. This antibody does not cross-react with LAP 2. MA1-074 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects 3 distinct proteins of molecular weights ~65, ~56 and ~ 51 kDa representing LAP 1A, 1B, and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with MA1-074 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining. The MA1-074 immunogen is pore complex-lamina fraction isolated from rat liver nuclear envelopes.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RL13
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton.
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane.
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
Haque F, Lloyd DJ, Smallwood DT, Dent CL, Shanahan CM, Fry AM, Trembath RC, Shackleton S
Molecular and cellular biology 2006 May;26(10):3738-51
Molecular and cellular biology 2006 May;26(10):3738-51
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
Yang L, Guan T, Gerace L
The Journal of cell biology 1997 Dec 1;139(5):1077-87
The Journal of cell biology 1997 Dec 1;139(5):1077-87
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
Yang L, Guan T, Gerace L
The Journal of cell biology 1997 Dec 1;139(5):1077-87
The Journal of cell biology 1997 Dec 1;139(5):1077-87
cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane.
Martin L, Crimaudo C, Gerace L
The Journal of biological chemistry 1995 Apr 14;270(15):8822-8
The Journal of biological chemistry 1995 Apr 14;270(15):8822-8
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
Foisner R, Gerace L
Cell 1993 Jul 2;73(7):1267-79
Cell 1993 Jul 2;73(7):1267-79
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
Senior A, Gerace L
The Journal of cell biology 1988 Dec;107(6 Pt 1):2029-36
The Journal of cell biology 1988 Dec;107(6 Pt 1):2029-36
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LAP1 using anti-LAP1 monoclonal antibody (Product # MA1-074) shows staining in NS-1 Cells.
