Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30366 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaxin 6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain, rat brain. Predicted reactivity: Mouse (93%), Rat (94%), Pig (97%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Syntaxin 6 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Syntaxin 6 Polyclonal Antibody (Product # PA5-30366) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Syntaxin 6 Polyclonal Antibody (Product # PA5-30366). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. Syntaxin 6 Polyclonal Antibody (Product # PA5-30366) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Syntaxin 6 was achieved by transfecting SH-SY5Y with Syntaxin 6 specific siRNA (Silencer® select Product # s19959, s19958). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the Syntaxin 6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Syntaxin 6 Recombinant Polyclonal Antibody (Product # PA5-30366, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Syntaxin 6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Syntaxin 6 Polyclonal Antibody (Product # PA5-30366) and a 30kDa band corresponding to Syntaxin 6, along with non specific bands (*) were observed in the cell lines tested. Whole cell extracts (30 µg lysate) of SK-N-SH (Lane 1), SH-SY5Y (Lane 2), IMR-32 (Lane 3), HT-29 (Lane 4), Caco-2 (Lane 5) and MCF-7 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Syntaxin 6 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin 6 Polyclonal Antibody (Product # PA5 30366) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Syntaxin 6 (Product # PA5-30366) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.