361103

antibody from BioLegend

Targeting: MR1 HLALS

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

361103

Provider

BioLegend

Proper citation

BioLegend Cat#361103, RRID:AB_2563041

Product name

LEAF™ Purified anti-human/mouse/rat MR1

Antibody type

Monoclonal

Antigen

Soluble complexes of MR1 ectodomain and B2M.

Description

MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

26.5

Vial size

100 µg

Concentration

1.0 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human MR1-transfected Hela cell line was stained with LEAF™ purified MR1 (clone 26.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.

Conjugate

Unconjugated