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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-56287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IER5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RRNLEQPPSG GEDDDAEEME TGNVANLISI FGSSFSGLLR KSPGGGREEE EGEESGPEAA EPGQICCDKP V Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 77%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references High IER5 Gene Expression Is Associated With Poor Prognosis in Glioma Patients.
Wu Z, Wang D, Zeng F, Zhang Y, Zhu G, Ma Y, Song B, Lui S, Wu M
Frontiers in cell and developmental biology 2021;9:679684
Frontiers in cell and developmental biology 2021;9:679684
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IER5 in human cell line U-251 MG shows positivity in nucleus & nucleoli. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IER5 in mouse caudate putamen shows weak synaptic-like immunoreactivity. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IER5 in mouse hippocampus shows synaptic-like positivity. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IER5 in mouse olfactory bulb shows weak labeling in the external plexiform layer. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IER5 in mouse caudate putamen shows weak synaptic-like immunoreactivity. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IER5 in human cerebral cortex tissue shows moderate positivity in neuropil. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IER5 in human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells. Samples were probed using an IER5 Polyclonal Antibody (Product # PA5-56287).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Immunohistochemistry of IER5 expression in the glioma tissue microarray cohort and the validation of prognosis value of IER5 expression in Chinese Glioma Genome Atlas (CGGA) datasets. (A) Representative images of IER5 staining in the negative, weak, moderate, and strong expression groups. (B) Kaplan-Meier survival curves of overall survival stratified by IER5 protein expression. (C) Kaplan-Meier survival curves of disease-specific survival stratified by IER5 protein expression. (D-F) Kaplan-Meier survival curves of overall survival stratified by IER5 expression in three independent datasets of CGGA.
