Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-01584 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RGS16 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Hepatic arginase 2 (Arg2) is sufficient to convey the therapeutic metabolic effects of fasting.
Zhang Y, Higgins CB, Fortune HM, Chen P, Stothard AI, Mayer AL, Swarts BM, DeBosch BJ
Nature communications 2019 Apr 8;10(1):1587
Nature communications 2019 Apr 8;10(1):1587
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGS16 Antibody (4E5) [NBP2-01584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS16.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGS16 Antibody (4E5) [NBP2-01584] Analysis of extracts (35ug) from 9 different cell lines by using anti-RGS16 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human breast tissue using anti-RGS16 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human endometrium tissue using anti-RGS16 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human Kidney tissue using anti-RGS16 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RGS16 Antibody (4E5) [NBP2-01584] - Analysis of Jurkat cells, using anti-RGS16 antibody, (Red), compared to a nonspecific negative control antibody (Blue).