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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- APC-125-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir7.1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse kidney lysates (lanes 3 and 6): - 1-3. Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:200).4-6. Anti-Kir7.1 (extracellular) Antibody , preincubated with Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Kir7.1 in rat kidney - Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:100). Staining is present in both distal (DT) and proximal (PT) tubules and in the collecting ducts (CD) in the renal cortex. Note that staining is absent both inglomeruli (Gl) and blood vessels (arrow). Hematoxilin is used as the counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Kir7.1 in live intact Jurkat (human T cell leukemia) cell line: - (black) Cells + Goat- Anti-Rabbit-FITC. (green) Cells + Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:20) + Goat- Anti-Rabbit-FITC.
