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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-30039 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NEU2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.52 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEU2 Antibody [NBP2-30039] - Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEU2 Antibody [NBP2-30039] - 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEU2 Antibody (RB32244) [NBP2-30039] - Anti-NEU2 Antibody (N-term) + Human heart tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. Predicted band size : 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NEU2 Antibody [NBP2-30039] - Confocal immunofluorescent analysis of (N-term)(NBP2-30039) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEU2 Antibody [NBP2-30039] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NEU2 Antibody [NBP2-30039] - Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
