ABIN954803

antibody from antibodies-online

Targeting: NEU2 SIAL2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN954803

Provider

antibodies-online

Product name

anti-Sialidase 2 (Cytosolic Sialidase) (NEU2) (N-Term), (AA 30-58) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of Human Sialidase-2 Genename: NEU2

Description

Protein A column, followed by peptide affinity purification

Reactivity

Human

Host

Rabbit

Epitope

N-Term,AA 30-58

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Defective myogenic differentiation of human rhabdomyosarcoma cells is characterized by sialidase Neu2 loss of expression.

Stoppani E, Rossi S, Marchesini S, Preti A, Fanzani A
Cell biology international 2009 Sep;33(9):1020-5

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A nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir.

Li CY, Yu Q, Ye ZQ, Sun Y, He Q, Li XM, Zhang W, Luo J, Gu X, Zheng X, Wei L
Cell research 2007 Apr;17(4):357-62

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Crystal structure of the human cytosolic sialidase Neu2. Evidence for the dynamic nature of substrate recognition.

Chavas LM, Tringali C, Fusi P, Venerando B, Tettamanti G, Kato R, Monti E, Wakatsuki S
The Journal of biological chemistry 2005 Jan 7;280(1):469-75

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Neu4, a novel human lysosomal lumen sialidase, confers normal phenotype to sialidosis and galactosialidosis cells.

Seyrantepe V, Landry K, Trudel S, Hassan JA, Morales CR, Pshezhetsky AV
The Journal of biological chemistry 2004 Aug 27;279(35):37021-9

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of NEU2 Antibody (N-term) Cat.-No AP52855PU-N in A549 cell line lysates (35ug/lane). This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of NEU2 Antibody (N-term) Cat.-No AP52855PU-N with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with NEU2 Antibody (N-term) Cat.-No AP52855PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of NEU2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Flow cytometric analysis of A549 cells using NEU2 Antibody (N-term) Cat.-No AP52855PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.