OMA1-06020

antibody from Invitrogen Antibodies

Targeting: SMTN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OMA1-06020

Provider

Invitrogen Antibodies

Product name

Smoothelin Monoclonal Antibody (R4A)

Antibody type

Monoclonal

Antigen

Other

Description

OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken and monkey tissues. This antibody detects both the visceral and vascular smoothelin isoforms. Does not cross react with rat samples. OMA1-06020 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~59 kDa protein representing visceral smoothelin and an ~100 kDa protein representing vascular smoothelin from canine smooth muscle extracts. This antibody can be used to discriminate fully differentiated (contractile) smooth muscle cells from proliferative smooth muscle cells. Staining of formalin-fixed paraffin-embedded tissues requires boiling in 10 mM citrate buffer (pH 6.0) for 3x5 min, prior to immunostaining. OMA1-06020 antigen is cytoskeletal smoothelin extract of chicken gizzard. Store at 4ºC, or in small aliquots at -20ºC.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Feline, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

R4A

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Palmitate induces glycosylation of cyclooxygenase-2 in primary human vascular smooth muscle cells.

Raman P, Madhavpeddi L, Gonzales RJ
American journal of physiology. Cell physiology 2018 May 1;314(5):C545-C553

---

Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.

Wamhoff BR, Bowles DK, Dietz NJ, Hu Q, Sturek M
American journal of physiology. Heart and circulatory physiology 2002 Dec;283(6):H2397-410

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Smoothelin Monoclonal Antibody (R4A) (Product # OMA1-06020) and a 100kDa band corresponding to Smoothelin was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of PC-3 (Lane 1), HeLa (Lane 2), Mouse Uterus (Lane 3), Mouse Brain (Lane 4) and Mouse Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:5000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of chicken gizzard striated muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on frozen section of chicken gizzard striated muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry on paraffin section of human smooth muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).