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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-62481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Smoothelin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: LAGLDEGALR KLLEVTADLA ERRRIRSAIR ELQRQELERE EEALASKRFR AERQDNKENW LHSQQREAEQ RAALARLAGQ LESMNDVEEL TALLR Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 96%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Smoothelin in human cell line U-2 OS shows positivity in actin filaments & nucleus but excluded from the nucleoli. Samples were probed using a Smoothelin Polyclonal Antibody (Product # PA5-62481).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Smoothelin in human cerebral cortex using Smoothelin Polyclonal Antibody (Product # PA5-62481) shows no positivity in neurons as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Smoothelin in human liver using Smoothelin Polyclonal Antibody (Product # PA5-62481) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Smoothelin in human prostate using Smoothelin Polyclonal Antibody (Product # PA5-62481) shows moderate cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Smoothelin in human smooth muscle and tonsil tissues using Smoothelin Polyclonal Antibody (Product # PA5-62481). Corresponding SMTN RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Smoothelin in human tonsil using Smoothelin Polyclonal Antibody (Product # PA5-62481) shows low expression as expected.
