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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-47606 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47606, RRID:AB_10010563
- Product name
- Mouse Monoclonal KCNT2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 140kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chloride intracellular channel 1 cooperates with potassium channel EAG2 to promote medulloblastoma growth.
Francisco MA, Wanggou S, Fan JJ, Dong W, Chen X, Momin A, Abeysundara N, Min HK, Chan J, McAdam R, Sia M, Pusong RJ, Liu S, Patel N, Ramaswamy V, Kijima N, Wang LY, Song Y, Kafri R, Taylor MD, Li X, Huang X
The Journal of experimental medicine 2020 May 4;217(5)
The Journal of experimental medicine 2020 May 4;217(5)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCNT2 Antibody (S11-33) [NBP1-47606] - analysis of Rat brain membrane lysate showing detection of Slo2.1 Potassium Channel protein using Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody, Clone S11-33 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606] - Tissue: backskin. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KCNT2 Antibody (S11-33) [NBP1-47606] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute.
