- PA5-18315 - Invitrogen Antibodies ABCC4 antibody

Submitted references Isothiocyanates (ITCs) 1-(Isothiocyanatomethyl)-4-phenylbenzene and 1-Isothiocyanato-3,5-bis(trifluoromethyl)benzene-Aldehyde Dehydrogenase (ALDH) Inhibitors, Decreases Cisplatin Tolerance and Migratory Ability of NSCLC.

Kryczka J, Kryczka J, Janczewski Ł, Gajda A, Frączyk A, Boncela J, Kolesińska B, Brzeziańska-Lasota E
International journal of molecular sciences 2022 Aug 3;23(15)

Implications of ABCC4-Mediated cAMP Eflux for CRC Migration.

Kryczka J, Sochacka E, Papiewska-Pająk I, Boncela J
Cancers 2020 Nov 27;12(12)

Knockdown of RAP2A gene expression suppresses cisplatin resistance in gastric cancer cells.

Zhang J, Wei Y, Min J, Wang Y, Yin L, Cao G, Shen H
Oncology letters 2020 Jan;19(1):350-358

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (PFA fixed) analysis of MRP4 using MRP4 Polyclonal Antibody (Product # PA5-18315) (4 µg/mL) in staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of MRP4 in human prostate. Samples were incubated with MRP4 polyclonal antibody (Product # PA5-18315) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 ABCC4 protein expression level in CRC cell lines. Western blot performed in standard reducing SDS PAGE conditions using goat anti ABCC4 (#PA5 18315, Thermo Scientific) and rabbit anti ABCG2 (#ORB 155559 Biorbyt). ( A ) Protein expression level of ABCC4 and ABCG2 in HT-29 stably overexpressing transcription factor Snail (HT29/Snail) and control HT-29. ABCC4 level in the membrane fraction (obtained by biotynylation using EZ-Link Sulfo -NHS-Biotin Thermo Scientific kit) of HT-29 control cells and HT-29 Snail n = 3. ( B ) ABCC4 protein expression level in CRC cells in different states of EMT: CCD841CoN (most epithelial), CaCo-2 (moderate EMT), and Colo-320 (most mesenchymal) n = 3. ( C ) ABCC4 protein abundance in Extracellular Vesicles (EVs) released from HT-29 control cells and two HT-29 stably overexpressing transcription factor Snail clones (HT-29/Snail and HT-29/Snail17), n = 2. ( D ) Intracellular cAMP level measurement. Accumulation of cAMP in HT29 cells was measured using a cAMP competitive kit (#581001 Cayman Chemical). Cells were incubated for 24 h with MK571 20 uM, or untreated ones were assayed according to the manufacturer's protocol. Calculation were conducted using the Cayman data sheet. cAMP concentration of HT29 was set as 100%. T -test performed, n = 5; * p < 0.05; ** p < 0.005; *** p < 0.001. NS--not statistically significant. ( E ) PKA phosphorylation profile analysis. HT29 Snail cells were seeded on a 6-well plate. Then, 24 h after, full growth mediu

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. DDP-resistant gastric cancer cells exhibit suppressed levels of apoptosis and DNA damage. (A) Percentages of apoptotic MGC803 and MGC803/DDP cells following DDP treatment. Cell apoptosis following DDP treatment (1.8 µg/mL for 48 h) was evaluated via flow cytometry. (B) DNA damage in MGC803 and MGC803/DDP cells treated with DDP. DNA damage following DDP treatment (1.8 µg/mL for 48 h) was analyzed by Hoechst 33342 staining and flow cytometry. (C) Levels of RAP2A, MRP and cleaved-caspase-3 in MGC803 and MGC803/DDP cells treated with DDP. Relative levels of protein expression were determined via western blotting, using GAPDH as an internal standard. **P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. RAP2A promotes migration, invasion and DDP resistance in MGC803/DDP cells. (A) RAP2A expression was knocked down by siRNA. (B and C) Levels of RAP2A, MRP and cleaved-caspase-3 in RAP2A-knockdown MGC803/DDP cells during DDP treatment. Relative levels of protein expression were determined via western blotting, with GAPDH serving as an internal standard. (D) Effect of RAP2A siRNA knockdown on MGC803/DDP cell viability during DDP treatment. Cell viability was measured using the Cell Counting Kit-8 assay. (E) Migration and invasion of MGC803/DDP cells with RAP2A siRNA knockdown during DDP treatment for 24 h (x100 magnification). Cell migration and invasion were analyzed using the Transwell system. Statistical analysis of MGC803/DDP cell migration and invasion. # P

PA5-18315

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