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LearnPA5-23885
antibody from Invitrogen Antibodies
Targeting: DENND1B
C1orf218, FAM31B, FLJ20054, MGC27044
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-23885 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DENND1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DENND1B in MOLT-4 whole cell lysate. Samples were incubated with DENND1B polyclonal antibody (Product # PA5-23885) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DENND1B in NCI-H460 cell line lysates. Samples were incubated with DENND1B polyclonal antibody (Product # PA5-23885). Lysates: 35 µg/lane. DENND1B protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DENND1B in formalin fixed and paraffin embedded human lymph tissue. Samples were incubated with DENND1B polyclonal antibody (Product # PA5-23885) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DENND1B in formalin fixed and paraffin embedded human lymph tissue. Samples were incubated with DENND1B polyclonal antibody (Product # PA5-23885) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H460 cells using a DENND1B polyclonal antibody (Product # PA5-23885) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of DENND1B in NCI-H460 cells (right histogram). Samples were incubated with DENND1B polyclonal antibody (Product # PA5-23885) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
