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- Antigen structure
- References [11]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 17056-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17056-1-AP, RRID:AB_2210295
- Product name
- UBE2F antibody
- Antibody type
- Polyclonal
- Description
- UBE2F antibody (Cat. #17056-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer Cells.
E3 Ligase Rbx1 Orchestrates Thymus Development and Fate Determination of αβ-γδ T Cells.
Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling.
RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis.
UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma.
Unbiased CRISPR Synthetic Lethal Screening for Genetic Vulnerabilities in Succinate Dehydrogenase (SDH)-loss Model of Paraganglioma.
The UBE2F-CRL5(ASB11)-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells.
Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis.
Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3(SPOP) E3 ligase in cancer cells.
The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness.
Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.
Cui D, Qu R, Li T, Wang L, Chen X, Shao S, Liang X, Xu J, Sun Y, Xiong X, Zhao Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(3):e12652
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(3):e12652
E3 Ligase Rbx1 Orchestrates Thymus Development and Fate Determination of αβ-γδ T Cells.
Wu D, Chen Q, Tan M, Sun Y
Research (Washington, D.C.) 2025;8:0774
Research (Washington, D.C.) 2025;8:0774
Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling.
Nikolic I, Cursons J, Shields B, Chappaz S, Sudholz H, Meng X, Constantinescu P, Vijayakumaran R, D'Angelo M, Foroutan M, Ladd D, Veldman M, Glab J, Procter T, Park HY, Contet J, Deuss F, Wong K, Sun Y, Berry R, Rautela J, Huntington ND
Cancer cell 2025 Nov 10;43(11):2034-2050.e11
Cancer cell 2025 Nov 10;43(11):2034-2050.e11
RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis.
Zhang F, Xiong X, Li Z, Wang H, Wang W, Zhao Y, Sun Y
The EMBO journal 2025 Feb;44(4):1185-1219
The EMBO journal 2025 Feb;44(4):1185-1219
UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma.
Xu L, Pan C, Wang Y, Ni L, Zeng P, Lin X, Lyu X, Huang S, Dang N
Journal of translational medicine 2025 Dec 8;24(1):52
Journal of translational medicine 2025 Dec 8;24(1):52
Unbiased CRISPR Synthetic Lethal Screening for Genetic Vulnerabilities in Succinate Dehydrogenase (SDH)-loss Model of Paraganglioma.
Al Khazal FJ, Emch MJ, de Araujo Correia CM, Favier J, Hawse JR, Maher LJ
bioRxiv : the preprint server for biology 2025 Aug 28;
bioRxiv : the preprint server for biology 2025 Aug 28;
The UBE2F-CRL5(ASB11)-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells.
Chang Y, Chen Q, Li H, Xu J, Tan M, Xiong X, Sun Y
Developmental cell 2024 May 20;59(10):1317-1332.e5
Developmental cell 2024 May 20;59(10):1317-1332.e5
Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis.
Zhang Y, Du L, Wang C, Jiang Z, Duan Q, Li Y, Xie Z, He Z, Sun Y, Huang L, Lu L, Wen C
Signal transduction and targeted therapy 2024 Jan 15;9(1):18
Signal transduction and targeted therapy 2024 Jan 15;9(1):18
Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3(SPOP) E3 ligase in cancer cells.
Zhou Q, Lin W, Wang C, Sun F, Ju S, Chen Q, Wang Y, Chen Y, Li H, Wang L, Hu Z, Jin H, Wang X, Sun Y
Nature communications 2022 May 31;13(1):3034
Nature communications 2022 May 31;13(1):3034
The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness.
Wu D, Li H, Liu M, Qin J, Sun Y
Nature communications 2022 May 31;13(1):3021
Nature communications 2022 May 31;13(1):3021
Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.
Hong X, Li S, Li W, Xie M, Wei Z, Guo H, Wei W, Zhang S
Biochemical and biophysical research communications 2019 Jan 15;508(3):986-990
Biochemical and biophysical research communications 2019 Jan 15;508(3):986-990
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP(UBE2F antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human skin using 17056-1-AP(UBE2F antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The UBE2F antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human UBE2F. This antibody recognizes human,mouse,rat antigen. The UBE2F antibody has been validated for the following applications: ELISA, WB, IHC analysis.
