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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB17034 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB17034, RRID:AB_10629789
- Product name
- UBE2F polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of UBE2F.
- Antigen sequence
C-HLRDKEDFRNKVDD- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA
Molecular cell 2009 Feb 27;33(4):483-95
Molecular cell 2009 Feb 27;33(4):483-95
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE2F polyclonal antibody (Cat # PAB17034) (0.05 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE2F polyclonal antibody (Cat # PAB17034) staining of paraffin embedded human placenta.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
