679404

antibody from BioLegend

Targeting: UBA1 A1S9T, CFAP124, GXP1, POC20, UBE1, UBE1X

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

679404

Provider

BioLegend

Proper citation

BioLegend Cat#679404, RRID:AB_2566181

Product name

Alexa Fluor® 594 anti-E1 Ubiquitin Activating Enzyme

Antibody type

Monoclonal

Antigen

Mixture of human and mouse E1 protein.

Description

The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. The gene for E1 Ubiquitin activating enzyme complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

2G2.3.5

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for give minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) E1 Ubiquitin Activating Enzyme Antibody (clone 2G2.3.5) in a blocking buffer overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye