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LearnPA5-30199
antibody from Invitrogen Antibodies
Targeting: UBA1
A1S9T, CFAP124, GXP1, POC20, UBE1, UBE1X
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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- Product number
- PA5-30199 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (91%), Rat (93%), Rabbit (92%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UBE1 using 30 µg of A) IMR32 and B) U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a UBE1 polyclonal antibody (Product # PA5-30199) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE1 Polyclonal Antibody (Product # PA5-30199). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UBE1 Polyclonal Antibody (Product # PA5-30199) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
