Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- FAB1418A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glut1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Induction of HIF-1α by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation.
Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
Duette G, Pereyra Gerber P, Rubione J, Perez PS, Landay AL, Crowe SM, Liao Z, Witwer KW, Holgado MP, Salido J, Geffner J, Sued O, Palmer CS, Ostrowski M
mBio 2018 Sep 11;9(5)
mBio 2018 Sep 11;9(5)
Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
Palmer CS, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge DC, Maisa A, Hearps AC, Lewin SR, Landay A, Jaworowski A, McCune JM, Crowe SM
AIDS (London, England) 2014 Jan 28;28(3):297-309
AIDS (London, England) 2014 Jan 28;28(3):297-309
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 APC-conjugated Monoclonal Antibody (Catalog # FAB1418A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 APC-conjugated Monoclonal Antibody (Catalog # FAB1418A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.