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LearnMA5-31960
antibody from Invitrogen Antibodies
Targeting: SLC2A1
CSE, DYT18, DYT9, GLUT, GLUT1, HTLVR
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [4]
- Immunohistochemistry [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-31960 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SA0377
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.
Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.
Risha Y, Minic Z, Ghobadloo SM, Berezovski MV
Scientific reports 2020 Aug 11;10(1):13572
Scientific reports 2020 Aug 11;10(1):13572
Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.
Wang W, Kini A, Wang Y, Liu T, Chen Y, Vukmanic E, Emery D, Liu Y, Lu X, Jin L, Lee SJ, Scott P, Liu X, Dean K, Lu Q, Fortuny E, James R, Kaplan HJ, Du J, Dean DC
Cell reports 2019 Jul 30;28(5):1323-1334.e4
Cell reports 2019 Jul 30;28(5):1323-1334.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GLUT1 in Hela cells using a GLUT1 Monoclonal antibody (Product # MA5-31960) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GLUT1 in MCF-7 cells using a GLUT1 Monoclonal antibody (Product # MA5-31960) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GLUT1 in HepG2 cells using a GLUT1 Monoclonal antibody (Product # MA5-31960) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GLUT1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), 1:2000 dilution, for 45 minutes at room temperature (Panel a: Blue). Nuclei (Panel b:Green) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT1 of paraffin-embedded Human liver tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT1 of paraffin-embedded Mouse liver tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT1 of paraffin-embedded Human kidney tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT1 of paraffin-embedded Human breast carcinoma tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT1 of paraffin-embedded Mouse kidney tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of GLUT1 in Hela cells using a GLUT1 Monoclonal Antibody (Product # MA5-31960) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 tSNE plots of exosomes analyzed by FACS showing expression levels of ( a ) GPC-1 ( b ) GLUT-1 and ( c ) ADAM10 along with CD81 and CD63 on the surface of BC-derived exosomes.
