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LearnMA5-11315
antibody from Invitrogen Antibodies
Targeting: SLC2A1
CSE, DYT18, DYT9, GLUT, GLUT1, HTLVR
Immunocytochemistry
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- MA5-11315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GLUT1 Monoclonal Antibody (SPM498)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-11315 targets GLUT-1 in IHC (P) applications and shows reactivity with Human and Rat samples. The MA5-11315 immunogen is a synthetic peptide derived from C-terminal of human GLUT-1.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SPM498
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL.
Comparative study of expression and activity of glucose transporters between stem cell-derived brain microvascular endothelial cells and hCMEC/D3 cells.
Clinicopathological significance of glucose transporter protein-1 overexpression in human osteosarcoma.
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
Initial Experience With Gallium-68 DOTA-Octreotate PET/CT and Peptide Receptor Radionuclide Therapy for Pediatric Patients With Refractory Metastatic Neuroblastoma.
HIF-1α and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma.
High-risk human papillomavirus load and biomarkers in cervical intraepithelial neoplasia and cancer.
αB-crystallin: a novel regulator of breast cancer metastasis to the brain.
High FDG activity in focal fat necrosis: a pitfall in interpretation of posttreatment PET/CT in patients with non-Hodgkin lymphoma.
IMP3 and GLUT-1 immunohistochemistry for distinguishing benign from malignant mesothelial proliferations.
Tenascin-C, GLUT-1, and syndecan-2 expression in juvenile nasopharyngeal angiofibroma: correlations to vessel density and tumor stage.
Neuropilin-2 and vascular endothelial growth factor receptor-3 are up-regulated in human vascular malformations.
Prognostic assessment of hypoxia and metabolic markers in cervical cancer using automated digital image analysis of immunohistochemistry.
Transforming growth factor-β1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
Overexpression of GLUT1 correlates with Kras mutations in lung carcinomas.
Down-regulation of MutS homolog 3 by hypoxia in human colorectal cancer.
Carcinoma ex pleomorphic adenoma of the salivary glands: distinct clinicopathologic features and immunoprofiles between subgroups according to cellular differentiation.
Rajani RM, Ratelade J, Domenga-Denier V, Hase Y, Kalimo H, Kalaria RN, Joutel A
Acta neuropathologica communications 2019 Nov 21;7(1):187
Acta neuropathologica communications 2019 Nov 21;7(1):187
Comparative study of expression and activity of glucose transporters between stem cell-derived brain microvascular endothelial cells and hCMEC/D3 cells.
Al-Ahmad AJ
American journal of physiology. Cell physiology 2017 Oct 1;313(4):C421-C429
American journal of physiology. Cell physiology 2017 Oct 1;313(4):C421-C429
Clinicopathological significance of glucose transporter protein-1 overexpression in human osteosarcoma.
Fan J, Mei J, Zhang MZ, Yuan F, Li SZ, Yu GR, Chen LH, Tang Q, Xian CJ
Oncology letters 2017 Aug;14(2):2439-2445
Oncology letters 2017 Aug;14(2):2439-2445
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
Stebbins MJ, Wilson HK, Canfield SG, Qian T, Palecek SP, Shusta EV
Methods (San Diego, Calif.) 2016 May 15;101:93-102
Methods (San Diego, Calif.) 2016 May 15;101:93-102
Initial Experience With Gallium-68 DOTA-Octreotate PET/CT and Peptide Receptor Radionuclide Therapy for Pediatric Patients With Refractory Metastatic Neuroblastoma.
Kong G, Hofman MS, Murray WK, Wilson S, Wood P, Downie P, Super L, Hogg A, Eu P, Hicks RJ
Journal of pediatric hematology/oncology 2016 Mar;38(2):87-96
Journal of pediatric hematology/oncology 2016 Mar;38(2):87-96
HIF-1α and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma.
Kwon OJ, Park JJ, Ko GH, Seo JH, Jeong BK, Kang KM, Woo SH, Kim JP, Hwa JS, Carey TE
Head & neck 2015 Apr;37(4):505-10
Head & neck 2015 Apr;37(4):505-10
High-risk human papillomavirus load and biomarkers in cervical intraepithelial neoplasia and cancer.
Chang MS, Oh S, Jung EJ, Park JH, Jeon HW, Lee TS, Kim JH, Choi E, Byeon SJ, Park IA
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 May;122(5):427-36
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 May;122(5):427-36
αB-crystallin: a novel regulator of breast cancer metastasis to the brain.
Malin D, Strekalova E, Petrovic V, Deal AM, Al Ahmad A, Adamo B, Miller CR, Ugolkov A, Livasy C, Fritchie K, Hamilton E, Blackwell K, Geradts J, Ewend M, Carey L, Shusta EV, Anders CK, Cryns VL
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 1;20(1):56-67
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jan 1;20(1):56-67
High FDG activity in focal fat necrosis: a pitfall in interpretation of posttreatment PET/CT in patients with non-Hodgkin lymphoma.
Kashyap R, Lau E, George A, Seymour JF, Lade S, Hicks RJ, Hofman MS
European journal of nuclear medicine and molecular imaging 2013 Sep;40(9):1330-6
European journal of nuclear medicine and molecular imaging 2013 Sep;40(9):1330-6
IMP3 and GLUT-1 immunohistochemistry for distinguishing benign from malignant mesothelial proliferations.
Lee AF, Gown AM, Churg A
The American journal of surgical pathology 2013 Mar;37(3):421-6
The American journal of surgical pathology 2013 Mar;37(3):421-6
Tenascin-C, GLUT-1, and syndecan-2 expression in juvenile nasopharyngeal angiofibroma: correlations to vessel density and tumor stage.
Renkonen S, Heikkilä P, Haglund C, Mäkitie AA, Hagström J
Head & neck 2013 Jul;35(7):1036-42
Head & neck 2013 Jul;35(7):1036-42
Neuropilin-2 and vascular endothelial growth factor receptor-3 are up-regulated in human vascular malformations.
Partanen TA, Vuola P, Jauhiainen S, Lohi J, Salminen P, Pitkäranta A, Häkkinen SK, Honkonen K, Alitalo K, Ylä-Herttuala S
Angiogenesis 2013 Jan;16(1):137-46
Angiogenesis 2013 Jan;16(1):137-46
Prognostic assessment of hypoxia and metabolic markers in cervical cancer using automated digital image analysis of immunohistochemistry.
Kim BW, Cho H, Chung JY, Conway C, Ylaya K, Kim JH, Hewitt SM
Journal of translational medicine 2013 Aug 8;11:185
Journal of translational medicine 2013 Aug 8;11:185
Transforming growth factor-β1 may be a key mediator of the fibrogenic properties of neural cells in leprosy.
Petito RB, Amadeu TP, Pascarelli BM, Jardim MR, Vital RT, Antunes SL, Sarno EN
Journal of neuropathology and experimental neurology 2013 Apr;72(4):351-66
Journal of neuropathology and experimental neurology 2013 Apr;72(4):351-66
Overexpression of GLUT1 correlates with Kras mutations in lung carcinomas.
Sasaki H, Shitara M, Yokota K, Hikosaka Y, Moriyama S, Yano M, Fujii Y
Molecular medicine reports 2012 Mar;5(3):599-602
Molecular medicine reports 2012 Mar;5(3):599-602
Down-regulation of MutS homolog 3 by hypoxia in human colorectal cancer.
Li J, Koike J, Kugoh H, Arita M, Ohhira T, Kikuchi Y, Funahashi K, Takamatsu K, Boland CR, Koi M, Hemmi H
Biochimica et biophysica acta 2012 Apr;1823(4):889-99
Biochimica et biophysica acta 2012 Apr;1823(4):889-99
Carcinoma ex pleomorphic adenoma of the salivary glands: distinct clinicopathologic features and immunoprofiles between subgroups according to cellular differentiation.
Kim JW, Kwon GY, Roh JL, Choi SH, Nam SY, Kim SY, Cho KJ
Journal of Korean medical science 2011 Oct;26(10):1277-85
Journal of Korean medical science 2011 Oct;26(10):1277-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of GLUT-1 was done on NIH/3T3 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with GLUT-1 Mouse Monoclonal Antibody (MA511315, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
