PA5-28055

antibody from Invitrogen Antibodies

Targeting: GPC1 glypican

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28055

Provider

Invitrogen Antibodies

Product name

Glypican 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr). Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Characterization of nucleic acids from extracellular vesicle-enriched human sweat.

Bart G, Fischer D, Samoylenko A, Zhyvolozhnyi A, Stehantsev P, Miinalainen I, Kaakinen M, Nurmi T, Singh P, Kosamo S, Rannaste L, Viitala S, Hiltunen J, Vainio SJ
BMC genomics 2021 Jun 9;22(1):425

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (N3C3) (Product # PA5-28055) at concentration ranged from 7.5 to 240 ng/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 Polyclonal Antibody (Product # PA5-28055) at concentration ranged from 7.5 to 240 ng/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Glypican 1 was performed in MCF 7 cells fixed in ice cold MeOH for 5 min. Green: Glypican 1 Polyclonal Antibody (Product # PA5 28055) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Glypican 1 Polyclonal Antibody detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Glypican 1 stained by Glypican 1 Polyclonal Antibody (Product # PA5-28055) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Glypican 1 was performed in paraffin-embedded human normal pancreas (left) and pancreatic cancer (right) tissue using Glypican 1 Polyclonal Antibody (Product # PA5-28055) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TLR3 was performed in A431 cells using TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) (red) at a dilution of 1:100. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Glypican 1 was performed in MCF-7 whole cell extracts using 5 µg of Glypican 1 Polyclonal Antibody (Product # PA5-28055). Samples were transferred to a membrane and probed with Glypican 1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Sweat particles. Visual characterization of sweat particles. Panels A : TEM, thin sections of plastic embedded pelleted sweat from 4 donors number corresponding to sequencing library (Table 1 ). B : TEM, negative staining of ExoEasy isolated sweat EVs, C : NTA analysis of Exoeasy EVs, D : TEM, Immunostaining of isolated sweat EVs, E: western blot, protein from Exoeasy sweat preparations were stained with anti-CD63 antibody (ab193349). Full size western blot with region selected marked is shown as supplementary Fig. 6