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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-1533 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1533, RRID:AB_2165860
- Product name
- Goat Polyclonal POMC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
- Reactivity
- Human, Mouse, Rat, Porcine, Sheep
- Host
- Goat
- Antigen sequence
C Terminus- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Age-dependent decline of hypothalamic HIF2α in response to insulin and its contribution to advanced age-associated metabolic disorders in mice.
Hindbrain dorsal vagal complex AMPK controls hypothalamic gluco-regulatory transmitter and counter-regulatory hormone responses to hypoglycemia.
Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity.
Hindbrain 5'-Adenosine Monophosphate-activated Protein Kinase Mediates Short-term Food Deprivation Inhibition of the Gonadotropin-releasing Hormone-Luteinizing Hormone Axis: Role of Nitric Oxide.
Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2.
A potential role for the secretogranin II-derived peptide EM66 in the hypothalamic regulation of feeding behaviour.
ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling.
Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells.
Loss of autophagy in hypothalamic POMC neurons impairs lipolysis.
Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation.
Wang Z, Khor S, Cai D
The Journal of biological chemistry 2019 Mar 29;294(13):4946-4955
The Journal of biological chemistry 2019 Mar 29;294(13):4946-4955
Hindbrain dorsal vagal complex AMPK controls hypothalamic gluco-regulatory transmitter and counter-regulatory hormone responses to hypoglycemia.
Mandal SK, Briski KP
Brain research bulletin 2019 Jan;144:171-179
Brain research bulletin 2019 Jan;144:171-179
Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity.
Kim GH, Shi G, Somlo DR, Haataja L, Song S, Long Q, Nillni EA, Low MJ, Arvan P, Myers MG Jr, Qi L
The Journal of clinical investigation 2018 Mar 1;128(3):1125-1140
The Journal of clinical investigation 2018 Mar 1;128(3):1125-1140
Hindbrain 5'-Adenosine Monophosphate-activated Protein Kinase Mediates Short-term Food Deprivation Inhibition of the Gonadotropin-releasing Hormone-Luteinizing Hormone Axis: Role of Nitric Oxide.
Shakya M, Shrestha PK, Briski KP
Neuroscience 2018 Jul 15;383:46-59
Neuroscience 2018 Jul 15;383:46-59
Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2.
Klasvogt S, Zuschratter W, Schmidt A, Kröber A, Vorwerk S, Wolter R, Isermann B, Wimmers K, Rothkötter HJ, Nossol C
Cell death discovery 2017;3:17001
Cell death discovery 2017;3:17001
A potential role for the secretogranin II-derived peptide EM66 in the hypothalamic regulation of feeding behaviour.
Trebak F, Dubuc I, Arabo A, Alaoui A, Boukhzar L, Maucotel J, Picot M, Cherifi S, Duparc C, Leprince J, Prévost G, Anouar Y, Magoul R, Chartrel N
Journal of neuroendocrinology 2017 Mar;29(3)
Journal of neuroendocrinology 2017 Mar;29(3)
ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling.
Evans JF, Rodriguez S, Ragolia L
Cell and tissue research 2013 May;352(2):413-25
Cell and tissue research 2013 May;352(2):413-25
Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells.
Evans JF, Fernando A, Ragolia L
Molecular and cellular endocrinology 2012 May 15;355(1):60-70
Molecular and cellular endocrinology 2012 May 15;355(1):60-70
Loss of autophagy in hypothalamic POMC neurons impairs lipolysis.
Kaushik S, Arias E, Kwon H, Lopez NM, Athonvarangkul D, Sahu S, Schwartz GJ, Pessin JE, Singh R
EMBO reports 2012 Mar 1;13(3):258-65
EMBO reports 2012 Mar 1;13(3):258-65
Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation.
Mühlhäusler BS, Adam CL, Marrocco EM, Findlay PA, Roberts CT, McFarlane JR, Kauter KG, McMillen IC
The Journal of physiology 2005 May 15;565(Pt 1):185-95
The Journal of physiology 2005 May 15;565(Pt 1):185-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
