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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-102099 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LGR6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total LGR6.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease.
Emre C, Do KV, Jun B, Hjorth E, Alcalde SG, Kautzmann MI, Gordon WC, Nilsson P, Bazan NG, Schultzberg M
Acta neuropathologica communications 2021 Jun 29;9(1):116
Acta neuropathologica communications 2021 Jun 29;9(1):116
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGR6 in B16F10 cell lysate (left lane: treated with blocking peptide). Samples were incubated with LGR6 polyclonal antibody (Product # PA5-102099).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGR6 in HUVEC cells. Samples were incubated with LGR6 polyclonal antibody (Product # PA5-102099).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LGR6 in HUVEC cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with LGR6 polyclonal antibody (Product # PA5-102099) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Alterations in LM receptor levels . The levels of BLT1, ChemR23, LGR6, GPR18 and FPR2, receptors for pro-resolving lipid mediators (LMs), in cortex and hippocampus of WT and App KI mice aged 2, 4, 8 and 18 months were determined by Western blot analysis. ( a ) Representative blots and ( b ) quantitative analysis are shown. Results are mean +- SEM and analysed by Mann-Whitney U test ( # P < 0.05, ## P < 0.01, ### P < 0.001) for intergroup analysis. Kruskal-Wallis one-way analysis of variance test with Dunn's post hoc test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.001) was used for multiple comparisons between ages and genotypes. AA = arachidonic acid, BLT1 = leukotriene B4 receptor, ChemR23 = chemokine like receptor 1, DHA = docosahexaenoic acid, EPA = eicosapentaenoic acid, FPR2 = formyl peptide receptor 2, GPR18 = G-protein coupled receptor 18, LGR6 = leucine-rich repeat containing G-protein coupled receptor 6
