Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-23237 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR35 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of GPR35 Preserves Mitochondrial Function After Myocardial Infarction by Targeting Calpain 1/2.
Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages.
Chen K, He L, Li Y, Li X, Qiu C, Pei H, Yang D
Journal of cardiovascular pharmacology 2020 Jun;75(6):556-563
Journal of cardiovascular pharmacology 2020 Jun;75(6):556-563
Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages.
Sharmin O, Abir AH, Potol A, Alam M, Banik J, Rahman AFMT, Tarannum N, Wadud R, Habib ZF, Rahman M
Scientific reports 2020 Jun 10;10(1):9400
Scientific reports 2020 Jun 10;10(1):9400
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G Protein-Coupled Receptor GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW using a G Protein-Coupled Receptor GPR35 polyclonal antibody (Product # PA5-23237) at 0.5 µg/mL and 1.0 µg/mL, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GPR35 Polyclonal Antibody (Product # PA5-23237) and a 34 kDa band corresponding to G-protein coupled receptor 35 was observed across the cell lines tested except NIH:OVCAR-3. 5. Membrane enriched extracts (30 µg lysate) of HT-29 (Lane 1), Hep G2 (Lane 2), Caco-2 (Lane 3), U-937 (Lane 4), HCT 116 (Lane 5) and NIH:OVCAR-3 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (at a concentration1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW. Samples were incubated in GPR35 polyclonal antibody (Product # PA5-23237) using a dilution of 0.5 µg/mL and 1.0 µg/mL, respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPR35 in paraffin-embedded formalin-fixed MCF7 cells. Samples were incubated in GPR35 polyclonal antibody (Product # PA5-23237) using a dilution of 5 µg/mL. Isotype control (top left) and this antibody (bottom left, right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Cellular expression of GPR35 in the ischemic brain. ( A-D ) Represents the gating scheme for doublet discrimination and isolation of the singlet population. ( E ) Fluorescence minus one (FMO) control for Alexa fluor 488 labeled GPR35 positive cells. ( F ) Single stain control for Alexa fluor 488 labeled GPR35 positive cells. ( G-J ) Representation of the flowcytometry analysis revealing that the CD45 ( H ), CD11b ( I ), and Ly-6G ( J ) positive cells express GPR35 ( G ).