MA5-32249

antibody from Invitrogen Antibodies

Targeting: ADIPOR1 ACDCR1, PAQR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32249

Provider

Invitrogen Antibodies

Product name

Adiponectin Receptor 1 Recombinant Rabbit Monoclonal Antibody (SC69-04)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

SC69-04

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adiponectin Receptor 1 in human skeletal muscle lysates using a Adiponectin Receptor 1 Monoclonal antibody (Product # MA5-32249) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Adiponectin Receptor 1 Recombinant Rabbit Monoclonal Antibody (SC69-04) (Product # MA5-32249) and a 43 kDa band corresponding to Adiponectin Receptor 1 was observed across the cell lines and tissues tested except in thymus tissue. Membrane enriched extracts (30 µg lysate) of K-562 (Lane 1), SK-BR-3 (Lane 2), Raji (Lane 3), HeLa (Lane 4), tissue extracts of Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Thymus (Lane 7) and Rat Thymus (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adiponectin Receptor 1 in Hela cells using a Adiponectin Receptor 1 Monoclonal antibody (Product # MA5-32249) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adiponectin Receptor 1 in HepG2 cells using a Adiponectin Receptor 1 Monoclonal antibody (Product # MA5-32249) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adiponectin Receptor 1 in LO2 cells using a Adiponectin Receptor 1 Monoclonal antibody (Product # MA5-32249) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of Adiponectin Receptor 1 in HepG2 cells using a Adiponectin Receptor 1 Monoclonal Antibody (Product # MA5-32249) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.