PA1-059

antibody from Invitrogen Antibodies

Targeting: ADIPOR1 ACDCR1, PAQR1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-059

Provider

Invitrogen Antibodies

Product name

Adiponectin Receptor 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-059 detects human and rat adiponectin receptor 1. PA1-059 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~42 kDa protein corresponding to human adiponectin receptor 1 from transfected hepatocytes. The PA1-059 immunogen is a synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth.

Messaggio F, Mendonsa AM, Castellanos J, Nagathihalli NS, Gorden L, Merchant NB, VanSaun MN
Oncotarget 2017 Oct 17;8(49):85378-85391

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Increase of adiponectin receptor gene expression by physical exercise in soleus muscle of obese Zucker rats.

Chang SP, Chen YH, Chang WC, Liu IM, Cheng JT
European journal of applied physiology 2006 May;97(2):189-95

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adiponectin Receptor 1 was performed by loading 25 µg of rat skeletal muscle (lane 1), human skeletal muscle (lane 2) and HepG2 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with an Adiponectin Receptor 1 polyclonal antibody (Product # PA1-059) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~42 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Adiponectin receptor levels are reduced in pancreatic cancer Human and murine normal and pancreatic cancer samples were immunostained for ADIPOR1 and ADIPOR2. (A) strong staining is present in the acinar tissue of human normal samples for both receptors but is decreased in the human pancreatic tumor samples. (B) murine pancreatic cancer tissue samples from PKT mice demonstrate a high level of staining in the adjacent acinar tissue and a reduced staining in the dysplastic pancreatic tumor. Graphs represent quantitative analysis of mean fluorescence from single channel images. Scale bar is 100mum. (C-D) quantitative real time PCR analysis was used to determine the relative expression of AdipoR1 (white bars) and AdipoR2 (black bars) in human and murine pancreatic tumor samples (PDAC) as well as in pancreatic cancer cell lines, relative to the level expressed in the normal pancreas (NP) tissue. (E) quantitative real time PCR analysis for adiponectin expression in human and mouse pancreatic cancer cell lines and mouse tissue, relative to human adipose tissue (AT), 3T3L1 cells differentiated to adipocytes (3T3L1) and mouse AT, respectively. Statistical analysis was performed separately for ADIPOR1 and ADIPOR2 protein levels (unpaired t-test) as well as AdipoR1 and AdipoR2 expression (two-way Anova), or adiponectin expression (ordinary one-way Anova) (*P