ALX-210-913-R100

antibody from Enzo Life Sciences

Targeting: ADIPOR1 ACDCR1, PAQR1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ALX-210-913-R100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-210-913-R100, RRID:AB_2050650

Product name

Adiponectin receptor 1 (human) polyclonal antibody (AL238)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Vial size

100 μl

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of Adiponectin Receptor 1 (human) in HEK 293T transfected cells by the PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913). Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lanes 1 and 3) or HEK 293T cells transfected with an expression vector encoding the Flag-tagged human AdipoR1 (lanes 2 and 4) were run on a 12% SDS-PAGE gel and analyzed by Western blot. Lanes 1 and 2 were probed with an anti-FLAG antibody at a 1:2'000 dilution. Lanes 3 and 4 were probed with AL238 at a 1:1'000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with goat anti-mouse horseradish peroxydase (Prod. No. APO-26G-050H) or a goat anti-rabbit horseradish peroxydase (Jackson, Prod. No. 111-035-144), respectively, for ECL detection. Note that the upper bands might represent oligomerized forms of the receptor.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Paraffin embedded human adipose tissue stained with PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913).