MA5-26185

antibody from Invitrogen Antibodies

Targeting: MYBPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-26185

Provider

Invitrogen Antibodies

Product name

MYBPH Monoclonal Antibody (OTI3G1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3G1

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Myogenic regulatory factors are key players in determining muscle mass and meat quality in Jeju native and Berkshire pigs.

Kim K, Kim D, Min Y, Jeong D, Son YO, Do K
Veterinary medicine and science 2021 May;7(3):735-745

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MYBPH in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MYBPH (Product # MA5-26185) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MYBPH in COS7 cells. Cells were transfected with a plasmid overexpressing MYBPH and probed with a MYBPH monoclonal antibody (Product # MA5-26185).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 Protein levels of myogenic regulatory factors (MRFs). The protein levels of MyoD, Pax7, Myf5, Myf6 and MyBPH were analysed by western blotting using the longissimus dorsi muscles of 1-day-old piglets and adult pigs of Berkshire and JNPs (a). Protein levels were normalized to beta-actin, and relative intensity of MyoD (b), Pax7 (c), Myf5 (d), Myf6 (e) and MyBPH (f) are represented. Values are presented as mean +- standard error of mean (s.e.m.) (* p < .05, ** p < .01 and *** p < .001; two-tailed t -test). JNP: Jeju native pig