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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- TA500614 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500614, RRID:AB_2284289
- Product name
- PPP1R7 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)
- Antibody type
- Monoclonal
- Description
- PPP1R7 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PPP1R7
- Isotype
- IgG
- Antibody clone number
- OTI4F9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references The Fra-1-miR-134-SDS22 feedback loop amplifies ERK/JNK signaling and reduces chemosensitivity in ovarian cancer cells.
Wu J, Sun Y, Zhang PY, Qian M, Zhang H, Chen X, Ma D, Xu Y, Chen X, Tang KF
Cell death & disease 2016 Sep 29;7(9):e2384
Cell death & disease 2016 Sep 29;7(9):e2384
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R7 (RC201634, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R7.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPP1R7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-PPP1R7 mouse monoclonal antibody (TA500614).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PPP1R7 mouse monoclonal antibody (TA500614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R7(RC201634).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP1R7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500614)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500614)
- Validation comment
- IHC
