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- References [2]
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- Immunohistochemistry [1]
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- Product number
- MAB974 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TIMP-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIMP-4 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human TIMP-1, -2, -3, or recombinant mouse TIMP-1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi.
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Drewlo S, Czikk M, Baczyk D, Lye S, Kingdom J
Human reproduction (Oxford, England) 2011 May;26(5):1025-34
Human reproduction (Oxford, England) 2011 May;26(5):1025-34
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Experimental hematology 2009 Jul;37(7):849-56
Experimental hematology 2009 Jul;37(7):849-56
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- TIMP-4 in Human Breast Cancer Tissue. TIMP-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human TIMP-4 Monoclonal Antibody (Catalog # MAB974) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
