LS-C744845

antibody from LSBio

Targeting: TIMP4

Western blot

ELISA

Antibody data

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Validation data

Product number: LS-C744845 - Provider product page
Provider: LSBio
Product name: TIMP4 Antibody (clone 9:4-7) LS-C744845
Antibody type: Monoclonal
Description: Protein A affinity chromatography
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 9:4-7
Storage: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

TIMP4 protein structure - LS-C744845 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMIP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TMIP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of Mouse anti-TIMP4 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMIP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TMIP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Mouse anti-TIMP4 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of Mouse Anti-TIMP4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) TIMP-4 antibody; and c) TIMP-4 each at 10 µg/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.