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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 14634-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14634-1-AP, RRID:AB_2300036
- Product name
- PPP1R15B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PPP1R15B antibody (Cat. #14634-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Stress response silencing by an E3 ligase mutated in neurodegeneration.
Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions.
Recruitment of trimeric eIF2 by phosphatase non-catalytic subunit PPP1R15B.
Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome.
The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acids.
UVB-induced eIF2α phosphorylation in keratinocytes depends on decreased ATF4, GADD34 and CReP expression levels.
CReP mediates selective translation initiation at the endoplasmic reticulum.
AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation.
Preemptive Activation of the Integrated Stress Response Protects Mice From Diet-Induced Obesity and Insulin Resistance by Fibroblast Growth Factor 21 Induction.
Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.
Chronically stressed or stress-preconditioned neurons fail to maintain stress granule assembly.
Nuclear Matrix Protein 4 Is a Novel Regulator of Ribosome Biogenesis and Controls the Unfolded Protein Response via Repression of Gadd34 Expression.
An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.
Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation.
Ribosome Reinitiation Directs Gene-specific Translation and Regulates the Integrated Stress Response.
Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability.
DNA Damage Regulates Translation through β-TRCP Targeting of CReP.
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
IRE1α-Dependent Decay of CReP/Ppp1r15b mRNA Increases Eukaryotic Initiation Factor 2α Phosphorylation and Suppresses Protein Synthesis.
Eukaryotic initiation factor 2α phosphorylation mediates fetal hemoglobin induction through a post-transcriptional mechanism.
A subunit of eukaryotic translation initiation factor 2α-phosphatase (CreP/PPP1R15B) regulates membrane traffic.
Haakonsen DL, Heider M, Ingersoll AJ, Vodehnal K, Witus SR, Uenaka T, Wernig M, Rapé M
Nature 2024 Feb;626(8000):874-880
Nature 2024 Feb;626(8000):874-880
Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions.
Misra J, Carlson KR, Spandau DF, Wek RC
Nucleic acids research 2024 Feb 28;52(4):1830-1846
Nucleic acids research 2024 Feb 28;52(4):1830-1846
Recruitment of trimeric eIF2 by phosphatase non-catalytic subunit PPP1R15B.
Fatalska A, Hodgson G, Freund SMV, Maslen SL, Morgan T, Thorkelsson SR, van Slegtenhorst M, Lorenz S, Andreeva A, Kaat LD, Bertolotti A
Molecular cell 2024 Feb 1;84(3):506-521.e11
Molecular cell 2024 Feb 1;84(3):506-521.e11
Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome.
Hooshmandi M, Sharma V, Thörn Perez C, Sood R, Krimbacher K, Wong C, Lister KC, Ureña Guzmán A, Bartley TD, Rocha C, Maussion G, Nadler E, Roque PM, Gantois I, Popic J, Lévesque M, Kaufman RJ, Avoli M, Sanz E, Nader K, Hagerman RJ, Durcan TM, Costa-Mattioli M, Prager-Khoutorsky M, Lacaille JC, Martinez-Cerdeno V, Gibson JR, Huber KM, Sonenberg N, Gkogkas CG, Khoutorsky A
Neuron 2023 Oct 4;111(19):3028-3040.e6
Neuron 2023 Oct 4;111(19):3028-3040.e6
The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acids.
Miles RR, Amin PH, Diaz MB, Misra J, Aukerman E, Das A, Ghosh N, Guith T, Knierman MD, Roy S, Spandau DF, Wek RC
The Journal of biological chemistry 2021 Nov;297(5):101257
The Journal of biological chemistry 2021 Nov;297(5):101257
UVB-induced eIF2α phosphorylation in keratinocytes depends on decreased ATF4, GADD34 and CReP expression levels.
Bahamondes Lorca VA, Bastidas Mayorga BD, Tong L, Wu S
Life sciences 2021 Dec 1;286:120044
Life sciences 2021 Dec 1;286:120044
CReP mediates selective translation initiation at the endoplasmic reticulum.
Kastan JP, Dobrikova EY, Bryant JD, Gromeier M
Science advances 2020 Jun;6(23):eaba0745
Science advances 2020 Jun;6(23):eaba0745
AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation.
Hülsmann J, Kravic B, Weith M, Gstaiger M, Aebersold R, Collins BC, Meyer H
Molecular & cellular proteomics : MCP 2018 Jul;17(7):1295-1307
Molecular & cellular proteomics : MCP 2018 Jul;17(7):1295-1307
Preemptive Activation of the Integrated Stress Response Protects Mice From Diet-Induced Obesity and Insulin Resistance by Fibroblast Growth Factor 21 Induction.
Xu X, Krumm C, So JS, Bare CJ, Holman C, Gromada J, Cohen DE, Lee AH
Hepatology (Baltimore, Md.) 2018 Dec;68(6):2167-2181
Hepatology (Baltimore, Md.) 2018 Dec;68(6):2167-2181
Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.
Krzyzosiak A, Sigurdardottir A, Luh L, Carrara M, Das I, Schneider K, Bertolotti A
Cell 2018 Aug 23;174(5):1216-1228.e19
Cell 2018 Aug 23;174(5):1216-1228.e19
Chronically stressed or stress-preconditioned neurons fail to maintain stress granule assembly.
Shelkovnikova TA, Dimasi P, Kukharsky MS, An H, Quintiero A, Schirmer C, Buée L, Galas MC, Buchman VL
Cell death & disease 2017 May 11;8(5):e2788
Cell death & disease 2017 May 11;8(5):e2788
Nuclear Matrix Protein 4 Is a Novel Regulator of Ribosome Biogenesis and Controls the Unfolded Protein Response via Repression of Gadd34 Expression.
Young SK, Shao Y, Bidwell JP, Wek RC
The Journal of biological chemistry 2016 Jun 24;291(26):13780-8
The Journal of biological chemistry 2016 Jun 24;291(26):13780-8
An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.
De Gassart A, Bujisic B, Zaffalon L, Decosterd LA, Di Micco A, Frera G, Tallant R, Martinon F
Proceedings of the National Academy of Sciences of the United States of America 2016 Jan 12;113(2):E117-26
Proceedings of the National Academy of Sciences of the United States of America 2016 Jan 12;113(2):E117-26
Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation.
Friend K, Brooks HA, Propson NE, Thomson JA, Kimble J
PloS one 2015;10(9):e0139076
PloS one 2015;10(9):e0139076
Ribosome Reinitiation Directs Gene-specific Translation and Regulates the Integrated Stress Response.
Young SK, Willy JA, Wu C, Sachs MS, Wek RC
The Journal of biological chemistry 2015 Nov 20;290(47):28257-28271
The Journal of biological chemistry 2015 Nov 20;290(47):28257-28271
Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability.
Kernohan KD, Tétreault M, Liwak-Muir U, Geraghty MT, Qin W, Venkateswaran S, Davila J, Care4Rare Canada Consortium, Holcik M, Majewski J, Richer J, Boycott KM
Human molecular genetics 2015 Nov 15;24(22):6293-300
Human molecular genetics 2015 Nov 15;24(22):6293-300
DNA Damage Regulates Translation through β-TRCP Targeting of CReP.
Loveless TB, Topacio BR, Vashisht AA, Galaang S, Ulrich KM, Young BD, Wohlschlegel JA, Toczyski DP
PLoS genetics 2015 Jun;11(6):e1005292
PLoS genetics 2015 Jun;11(6):e1005292
Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.
Wu H, Wei L, Fan F, Ji S, Zhang S, Geng J, Hong L, Fan X, Chen Q, Tian J, Jiang M, Sun X, Jin C, Yin ZY, Liu Q, Zhang J, Qin F, Lin KH, Yu JS, Deng X, Wang HR, Zhao B, Johnson RL, Chen L, Zhou D
Nature communications 2015 Feb 19;6:6239
Nature communications 2015 Feb 19;6:6239
IRE1α-Dependent Decay of CReP/Ppp1r15b mRNA Increases Eukaryotic Initiation Factor 2α Phosphorylation and Suppresses Protein Synthesis.
So JS, Cho S, Min SH, Kimball SR, Lee AH
Molecular and cellular biology 2015 Aug;35(16):2761-70
Molecular and cellular biology 2015 Aug;35(16):2761-70
Eukaryotic initiation factor 2α phosphorylation mediates fetal hemoglobin induction through a post-transcriptional mechanism.
Hahn CK, Lowrey CH
Blood 2013 Jul 25;122(4):477-85
Blood 2013 Jul 25;122(4):477-85
A subunit of eukaryotic translation initiation factor 2α-phosphatase (CreP/PPP1R15B) regulates membrane traffic.
Kloft N, Neukirch C, von Hoven G, Bobkiewicz W, Weis S, Boller K, Husmann M
The Journal of biological chemistry 2012 Oct 12;287(42):35299-35317
The Journal of biological chemistry 2012 Oct 12;287(42):35299-35317
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14634-1-AP(PPP1R15B;CReP antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The PPP1R15B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PPP1R15B. This antibody recognizes human,mouse,rat antigen. The PPP1R15B antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-PPP1R15B;CReP (IP:14634-1-AP, 3ug; Detection:14634-1-AP 1:1000) with MCF-7 cells lysate 2500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 14634-1-AP(PPP1R15B;CReP antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 14634-1-AP(PPP1R15B;CReP antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
