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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8020 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPP1R15B Antibody / Protein phosphatase 1 regulatory subunit 15B
- Antibody type
- Polyclonal
- Description
- This highly specific PPP1R15B antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PPP1R15B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) 293T, 2) HeLa and 3) MCF7 cell lysate with PPP1R15B antibody. Predicted molecular weight ~79 kDa but may be observed at up to ~100 kDa, possibly due to phosphorylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with PPP1R15B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human MCF7 cells with PPP1R15B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP1R15B antibody.
