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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF507 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal BMP-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BMP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) BMP-7 is observed and less than 1% cross-reactivity with rhBMP-2, rhBMP-4, rhBMP-5mature, rhBMP-8A, and recombinant mouse BMP-6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration.
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, Drechsel DN, Tanaka EM
Developmental cell 2017 Mar 27;40(6):608-617.e6
Developmental cell 2017 Mar 27;40(6):608-617.e6
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Chen J, Liu H, Liu J, Qi J, Wei B, Yang J, Liang H, Chen Y, Chen J, Wu Y, Guo L, Zhu J, Zhao X, Peng T, Zhang Y, Chen S, Li X, Li D, Wang T, Pei D
Nature genetics 2013 Jan;45(1):34-42
Nature genetics 2013 Jan;45(1):34-42
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Herrera B, Inman GJ
BMC cell biology 2009 Mar 19;10:20
BMC cell biology 2009 Mar 19;10:20
Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer research 2005 Sep 15;65(18):8274-85
Cancer research 2005 Sep 15;65(18):8274-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- BMP-6 in Human Lung. BMP-6 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF507) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-6 and Neutralization by Human BMP-6 Antibody. Recombinant Human BMP-6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-6 (0.15 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF507). The ND50 is typically 0.75-3.75 μg/ml in the presence of L-ascorbic acide (50 μg/mL).
