Explore
Validate
Learn
Western blot
Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP-6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) BMP-7 is observed and less than 1% cross-reactivity with rhBMP-2, rhBMP-4, rhBMP-5mature, rhBMP-8A, and recombinant mouse BMP-6 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BMP-6 in immersion fixed paraffin-embedded sections of human lung. Samples were incubated in BMP-6 polyclonal antibody (Product # PA5-47695) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle in vasculature.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of BMP-6 in ATDC5 mouse chondrogenic cell line. Samples were incubated in BMP-6 polyclonal antibody (Product # PA5-47695). Recombinant Human BMP‚6 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‚6 (0.15 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.75-3.75 μg/mL in the presence of L-ascorbic acide (50 μg/mL).
