PA5-47695

antibody from Invitrogen Antibodies

Targeting: BMP6 VGR, VGR1

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47695

Provider

Invitrogen Antibodies

Product name

BMP-6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) BMP-7 is observed and less than 1% cross-reactivity with rhBMP-2, rhBMP-4, rhBMP-5mature, rhBMP-8A, and recombinant mouse BMP-6 is observed.

Concentration

0.2 mg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMP-6 in immersion fixed paraffin-embedded sections of human lung. Samples were incubated in BMP-6 polyclonal antibody (Product # PA5-47695) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle in vasculature.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of BMP-6 in ATDC5 mouse chondrogenic cell line. Samples were incubated in BMP-6 polyclonal antibody (Product # PA5-47695). Recombinant Human BMP‚6 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‚6 (0.15 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.75-3.75 μg/mL in the presence of L-ascorbic acide (50 μg/mL).