PA5-47695

antibody from Invitrogen Antibodies

Targeting: BMP6 VGR, VGR1

Western blot (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47695

Provider

Invitrogen Antibodies

Product name

BMP-6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) BMP-7 is observed and less than 1% cross-reactivity with rhBMP-2, rhBMP-4, rhBMP-5mature, rhBMP-8A, and recombinant mouse BMP-6 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of BMP-6 in ATDC5 mouse chondrogenic cell line. Samples were incubated in BMP-6 polyclonal antibody (Product # PA5-47695). Recombinant Human BMP‚6 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‚6 (0.15 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.75-3.75 μg/mL in the presence of L-ascorbic acide (50 μg/mL).