A300-109A

antibody from Invitrogen Antibodies

Targeting: RBBP5 RBQ3, SWD1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-109A

Provider

Invitrogen Antibodies

Product name

RbBP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse RbBP5 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody (Product # A300-109A; lot A300-109A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RbBP5 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody (Product # A300-109A; lot A300-109A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RbBP5 by western blot. Sample: Whole cell lysate (50 µg for WB and Input; one 10 cm plate for IP) from 293T cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody A300-109A used at the indicated concentrations for WB or 10 µg for IP (15% of IP loaded). immunoprecipitatesd RbBP5 was detected by WB using 0.2 mg/ml. Detection: Chemiluminescence with 1 second (WB) or 10 second (IP/WB) exposure.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RbBP5 (Product # A300-109A lot 3) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 (Cat. No. A300-109A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP-chip scatter plot of anti-RbBP5 (A300-109A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-109A was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RbBP5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RbBP5 antibody (Product # A300-109A; lot A300-109A-3) used for IP at 6 µg per reaction. RbBP5 was also immunoprecipitated by a previous lot of this antibody (lot A300-109A-2). For blotting immunoprecipitated RbBP5 (Product # A300-109A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.