PA5-121050

antibody from Invitrogen Antibodies

Targeting: RBBP5 RBQ3, SWD1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121050

Provider

Invitrogen Antibodies

Product name

RBBP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: MCF7, NCI-H460, HeLa, Mouse spleen, Mouse brain, Mouse thymus, Rat liver. The target is usually found in the following locations: Nucleus. Immunogen sequence: SIWAQNQVEN WSAFAPDFKE LDENVEYEER ESEFDIEDED KSEPEQTGAD AAEDEEVDVT SVDPIAAFCS SDEELEDSKA LLYLPIAPEV EDPEENPYGP PPDAVQTSLM DEGASSEKKR QSSADGSQPP KKKPKTTNIE LQGVPNDEVH PLLGVKGDGK SKKKQAGRPK GSKGKEKDSP FKPKLYKGDR GLPLEGSAKG KVQAELSQPL TAGGAISELL

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.54 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBBP5 in extracts of various cell lines. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RBBP5 in A-549 cells. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RBBP5 in 300 μg extracts of MCF7 cells. Samples were precipitated with 3 μg RBBP5 Polyclonal antibody (Product # PA5-121050). Western blot was performed from the immunoprecipitate using RBBP5 Polyclonal antibody (Product # PA5-121050) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RBBP5 in paraffin-embedded rat Intestine. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RBBP5 in paraffin-embedded human colon carcinoma. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RBBP5 in paraffin-embedded mouse liver. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RBBP5 in paraffin-embedded human colon carcinoma. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RBBP5 in paraffin-embedded mouse liver. Samples were incubated with RBBP5 Polyclonal antibody (Product # PA5-121050) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.