HPA044140

antibody from Atlas Antibodies

Targeting: DSTYK DustyPK, KIAA0472, RIP5, RIPK5, SPG23

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA044140

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA044140, RRID:AB_10965031

Product name

Anti-DSTYK

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KENELYESLMNIANRKQEEMKDMIVETLNTMKEEL
LDDATNMEFKDVIVPENGEPVGTREIKCCIRQIQE
LIISRLNQAVANKLISSVDYLRESFVGTLERCLQS
LEKSQDVSVHITSNYLKQILNAAYHVEVTFHSGSS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-DSTYK antibody. Corresponding DSTYK RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and DSTYK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404577).

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and leydig cells).

Sample type

HUMAN