Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-92336 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92336, RRID:AB_11027354
- Product name
- Rabbit Polyclonal RIPK5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human RIPK5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Large Intragenic Deletion in DSTYK Underlies Autosomal-Recessive Complicated Spastic Paraparesis, SPG23.
Lee JYW, Hsu CK, Michael M, Nanda A, Liu L, McMillan JR, Pourreyron C, Takeichi T, Tolar J, Reid E, Hayday T, Blumen SC, Abu-Mouch S, Straussberg R, Basel-Vanagaite L, Barhum Y, Zouabi Y, Al-Ajmi H, Huang HY, Lin TC, Akiyama M, Lee JYY, McLean WHI, Simpson MA, Parsons M, McGrath JA
American journal of human genetics 2017 Feb 2;100(2):364-370
American journal of human genetics 2017 Feb 2;100(2):364-370
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK5 Antibody [NBP1-92336] - Analysis in human cell lines SK-MEL-30 and Caco-2 using anti-DSTYK antibody. Corresponding DSTYK RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK5 Antibody [NBP1-92336] - Analysis in control (vector only transfected HEK293T lysate) and DSTYK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK5 Antibody [NBP1-92336] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and leydig cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK5 Antibody [NBP1-92336] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK5 Antibody [NBP1-92336] - Staining of human kidney shows strong positivity in cells in tubules and glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK5 Antibody [NBP1-92336] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK5 Antibody [NBP1-92336] - Staining of human testis shows strong positivity in cytoplasm in cells in seminiferous ducts.