Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3168 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3168, RRID:AB_1581067
- Product name
- STK25 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of STK25.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways.
Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway.
Osada S, Izawa M, Saito R, Mizuno K, Suzuki A, Hirai S, Ohno S
Oncogene 1997 May 1;14(17):2047-57
Oncogene 1997 May 1;14(17):2047-57
Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway.
Pombo CM, Bonventre JV, Molnar A, Kyriakis J, Force T
The EMBO journal 1996 Sep 2;15(17):4537-46
The EMBO journal 1996 Sep 2;15(17):4537-46
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of STK25 polyclonal antibody (Cat # PAB3168) in A-549 cell lysate. STK25 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with STK25 polyclonal antibody (Cat # PAB3168) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)