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Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-34612 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUC5AC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with gorilla based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.9 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Interleukin-13 maintains the stemness of conjunctival epithelial cell cultures prepared from human limbal explants.
Stadnikova A, Trosan P, Skalicka P, Utheim TP, Jirsova K
PloS one 2019;14(2):e0211861
PloS one 2019;14(2):e0211861
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig 5 Immunofluorescent staining of MUC5AC and relative expression of MUC5AC and MUC4 genes in IL-13- or IL-13+ cell cultures. (A) P0 cells originating from limbal explants, P1 and P2 cells (all fixed at the end of cultivation), and the unattached GC-enriched subpopulation (P1d1, harvested on day 1 after passage of primary cells) were analyzed by immunofluorescent staining for MUC5AC (green). Nuclei were counterstained with DAPI (blue). (B) MUC5AC staining of GCs on pterygium cryosection (a) and upper bulbar conjunctival impression cytology (b). qPCR analysis of the relative gene expression of (C) MUC5AC and (D) MUC4 . All data are presented in vertical scatter plot graphs with line indicating median. Scale bars: 50 mum.
