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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
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- Product number
- MA1-19346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUC5AC Monoclonal Antibody (2-25LE)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals. A suggested positive control for this product is colorectal adenocarcinoma. For IHC (P), heat mediated antigen retrieval in sodium citrate is recommended.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2-25LE
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.
Manimala JC, Roach TA, Li Z, Gildersleeve JC
Glycobiology 2007 Aug;17(8):17C-23C
Glycobiology 2007 Aug;17(8):17C-23C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE) Monoclonal antibody (Product # MA1-19346).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE) Monoclonal antibody (Product # MA1-19346).
