AF4546
antibody from Novus Biologicals
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF4546 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SIRP alpha/CD172a Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human SIRP alpha/CD172a in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SIRP alpha/CD172a by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, A549 human lung carcinoma cell line, and U937 human histiocytic lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SIRP alpha/CD172a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4546) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SIRP alpha/CD172a at approximately 52 kDa (unglycosylated) and 90-120 kDa (glycosylated) as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SIRP alpha/CD172a by Simple WesternTM. Simple Western lane view shows lysates of U2OS human osteosarcoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SIRP alpha/CD172a at approximately 113-116 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human SIRP alpha/CD172a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4546) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.