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LearnMAB7307
antibody from R&D Systems
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB7307 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat SIRP alpha/CD172a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat SIRP-alpha/CD172a in direct ELISA and Western Blot.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P97710- Isotype
- IgG
- Antibody clone number
- 772734
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat SIRP alpha/CD172a by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Rat SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB7307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SIRP alpha/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of SIRP alpha/CD172a in PC-12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB7307, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Adhesion Induced by SIRP alpha/ CD172a and Neutralization by Rat SIRP alpha/CD172a Antibody. Recombinant Rat SIRP alpha/CD172a (Catalog # 7307-SA) supports the adhesion in mouse red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat SIRP alpha/CD172a (6 μg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat SIRP alpha/CD172a Monoclonal Antibody (Catalog # MAB7307). The ND50 is typically 0.04-0.16 μg/mL.
