H00140885-M01

antibody from Abnova Corporation

Targeting: SIRPA BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00140885-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00140885-M01, RRID:AB_606887

Product name

SIRPA monoclonal antibody (M01), clone 1D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant SIRPA.

Antigen sequence

VAGEEELQVIQPDKSVSVAAGESAILHCTVTSLIP
VGPIQWFRGAGPARELIYNQKEGHFPRVTTVSEST
KRENMDFSISISNITPADAGTYYCVKFRKGSPDTE
FKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSF
TCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGE
SVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGD
PLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTC
QVRKFYPQRLQLTWLENGNVSRTETASTVTENKDG
TYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSK
SHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGV
VCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEP
EKNAREITQVQSLDTNDITYADLNLPKGKKPAPQA
AEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLN
RTPKQPAPKPEPSFSEYASVQVPRK


Isotype

IgG

Antibody clone number

1D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SIRPA monoclonal antibody (M01), clone 1D10 Western Blot analysis of SIRPA expression in IMR-32 ( Cat # L008V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SIRPA is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol