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Learn17-1729-41
antibody from Invitrogen Antibodies
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 17-1729-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD172a (SIRP alpha) Monoclonal Antibody (15-414), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 15-414 monoclonal antibody reacts with human CD172a, also known as signal regulatory protein alpha (SIRP alpha). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, granulocytes, dendritic cells, and CD34+CD38- hematopoietic progenitor cells but not on T and B lymphocytes. Moreover, neurons have also been shown to express CD172. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.
- Antibody clone number
- 15-414
- Concentration
- 5 µL/Test
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice.
Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.
Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and function.
Signal regulatory protein (SIRPalpha), a cellular ligand for CD47, regulates neutrophil transmigration.
Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
FcαRI co-stimulation converts human intestinal CD103(+) dendritic cells into pro-inflammatory cells through glycolytic reprogramming.
Hansen IS, Krabbendam L, Bernink JH, Loayza-Puch F, Hoepel W, van Burgsteden JA, Kuijper EC, Buskens CJ, Bemelman WA, Zaat SAJ, Agami R, Vidarsson G, van den Brink GR, de Jong EC, Wildenberg ME, Baeten DLP, Everts B, den Dunnen J
Nature communications 2018 Feb 28;9(1):863
Nature communications 2018 Feb 28;9(1):863
Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.
Berry MR, Mathews RJ, Ferdinand JR, Jing C, Loudon KW, Wlodek E, Dennison TW, Kuper C, Neuhofer W, Clatworthy MR
Cell 2017 Aug 24;170(5):860-874.e19
Cell 2017 Aug 24;170(5):860-874.e19
Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
Balan S, Ollion V, Colletti N, Chelbi R, Montanana-Sanchis F, Liu H, Vu Manh TP, Sanchez C, Savoret J, Perrot I, Doffin AC, Fossum E, Bechlian D, Chabannon C, Bogen B, Asselin-Paturel C, Shaw M, Soos T, Caux C, Valladeau-Guilemond J, Dalod M
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Human hematopoietic reconstitution and HLA-restricted responses in nonpermissive alymphoid mice.
Serra-Hassoun M, Bourgine M, Boniotto M, Berges J, Langa F, Michel ML, Freitas AA, Garcia S
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1504-11
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1504-11
Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.
Abreu M, Sealy L
PloS one 2012;7(7):e41807
PloS one 2012;7(7):e41807
Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and function.
Barclay AN
Current opinion in immunology 2009 Feb;21(1):47-52
Current opinion in immunology 2009 Feb;21(1):47-52
Signal regulatory protein (SIRPalpha), a cellular ligand for CD47, regulates neutrophil transmigration.
Liu Y, Bühring HJ, Zen K, Burst SL, Schnell FJ, Williams IR, Parkos CA
The Journal of biological chemistry 2002 Mar 22;277(12):10028-36
The Journal of biological chemistry 2002 Mar 22;277(12):10028-36
Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells.
Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ
Blood 2001 May 1;97(9):2741-9
Blood 2001 May 1;97(9):2741-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD172a (SIRP alpha) APC (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
