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antibody from BioLegend
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [1]
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- Product number
- 323812 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#323812, RRID:AB_11219000
- Product name
- PerCP/Cy5.5 anti-human CD172a/b (SIRP/)
- Antibody type
- Monoclonal
- Antigen
- NIH-3T3/hu-SIRPα cell line
- Description
- CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells.2
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Far-Red dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood granulocytes were stained with anti-human CD172a/b (SIRPα) PerCP/Cy5.5 (clone SE5A5) (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).
