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LearnPA5-29544
antibody from Invitrogen Antibodies
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29544 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIRP alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HL-60, NIH-3T3.
- Concentration
- 0.24 mg/mL
Submitted references Genetic Mutation and Exosome Signature of Human Papilloma Virus Associated Oropharyngeal Cancer.
Kannan A, Hertweck KL, Philley JV, Wells RB, Dasgupta S
Scientific reports 2017 Apr 6;7:46102
Scientific reports 2017 Apr 6;7:46102
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIRP alpha using 30 µg of HL-60 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SIRP alpha polyclonal antibody (Product # PA5-29544) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIRP alpha using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SIRP alpha polyclonal antibody (Product # PA5-29544) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SIRP alpha Polyclonal Antibody (Product # PA5-29544). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRP alpha Polyclonal Antibody (Product # PA5-29544) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIRP alpha Polyclonal Antibody detects SIRP alpha protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIRP alpha stained by SIRP alpha Polyclonal Antibody (Product # PA5-29544) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody (Product # PA5-85158) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SIRP alpha (Product # PA5-29544) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
