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- References [17]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 12840-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12840-1-AP, RRID:AB_2135302
- Product name
- LGTN antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated LGTN antibody (Cat. #12840-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Translation of dipeptide repeat proteins in C9ORF72 ALS/FTD through unique and redundant AUG initiation codons.
Marine diterpenoid targets STING palmitoylation in mammalian cells.
The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D.
Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress.
Identification and characterization of novel proteins associated with CHD4.
Non-canonical initiation factors modulate repeat-associated non-AUG translation.
A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
Translation inhibitory elements from Hoxa3 and Hoxa11 mRNAs use uORFs for translation inhibition.
Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
Local translation in nuclear condensate amyloid bodies.
Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.
A viral RNA motif involved in signaling the initiation of translation on non-AUG codons.
The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells.
Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.
Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.
Sonobe Y, Lee S, Krishnan G, Gu Y, Kwon DY, Gao FB, Roos RP, Kratsios P
eLife 2023 Sep 7;12
eLife 2023 Sep 7;12
Marine diterpenoid targets STING palmitoylation in mammalian cells.
Hsiao WC, Niu GH, Lo CF, Wang JY, Chi YH, Huang WC, Tung CW, Sung PJ, Tsou LK, Zhang MM
Communications chemistry 2023 Jul 18;6(1):153
Communications chemistry 2023 Jul 18;6(1):153
The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D.
Kim H, Aponte-Diaz D, Sotoudegan MS, Shengjuler D, Arnold JJ, Cameron CE
PLoS biology 2023 Jan;21(1):e3001693
PLoS biology 2023 Jan;21(1):e3001693
Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress.
Makeeva DS, Riggs CL, Burakov AV, Ivanov PA, Kushchenko AS, Bykov DA, Popenko VI, Prassolov VS, Ivanov PV, Dmitriev SE
Cells 2023 Jan 8;12(2)
Cells 2023 Jan 8;12(2)
Identification and characterization of novel proteins associated with CHD4.
Sakaguchi C, Ichihara K, Nita A, Katayama Y, Nakayama KI
Genes to cells : devoted to molecular & cellular mechanisms 2022 Jan;27(1):61-71
Genes to cells : devoted to molecular & cellular mechanisms 2022 Jan;27(1):61-71
Non-canonical initiation factors modulate repeat-associated non-AUG translation.
Green KM, Miller SL, Malik I, Todd PK
Human molecular genetics 2022 Aug 17;31(15):2521-2534
Human molecular genetics 2022 Aug 17;31(15):2521-2534
A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
Sonobe Y, Aburas J, Krishnan G, Fleming AC, Ghadge G, Islam P, Warren EC, Gu Y, Kankel MW, Brown AEX, Kiskinis E, Gendron TF, Gao FB, Roos RP, Kratsios P
Nature communications 2021 Oct 15;12(1):6025
Nature communications 2021 Oct 15;12(1):6025
Translation inhibitory elements from Hoxa3 and Hoxa11 mRNAs use uORFs for translation inhibition.
Alghoul F, Laure S, Eriani G, Martin F
eLife 2021 Jun 2;10
eLife 2021 Jun 2;10
Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
Ichihara K, Matsumoto A, Nishida H, Kito Y, Shimizu H, Shichino Y, Iwasaki S, Imami K, Ishihama Y, Nakayama KI
Nucleic acids research 2021 Jul 21;49(13):7298-7317
Nucleic acids research 2021 Jul 21;49(13):7298-7317
Local translation in nuclear condensate amyloid bodies.
Theodoridis PR, Bokros M, Marijan D, Balukoff NC, Wang D, Kirk CC, Budine TD, Goldsmith HD, Wang M, Audas TE, Lee S
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)
Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.
Marmor-Kollet H, Siany A, Kedersha N, Knafo N, Rivkin N, Danino YM, Moens TG, Olender T, Sheban D, Cohen N, Dadosh T, Addadi Y, Ravid R, Eitan C, Toth Cohen B, Hofmann S, Riggs CL, Advani VM, Higginbottom A, Cooper-Knock J, Hanna JH, Merbl Y, Van Den Bosch L, Anderson P, Ivanov P, Geiger T, Hornstein E
Molecular cell 2020 Dec 3;80(5):876-891.e6
Molecular cell 2020 Dec 3;80(5):876-891.e6
A viral RNA motif involved in signaling the initiation of translation on non-AUG codons.
Sanz MA, Almela EG, García-Moreno M, Marina AI, Carrasco L
RNA (New York, N.Y.) 2019 Apr;25(4):431-452
RNA (New York, N.Y.) 2019 Apr;25(4):431-452
The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells.
González-Almela E, Williams H, Sanz MA, Carrasco L
Frontiers in microbiology 2018;9:207
Frontiers in microbiology 2018;9:207
Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S
Cell reports 2018 Jan 2;22(1):17-26
Cell reports 2018 Jan 2;22(1):17-26
Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.
Sanz MA, González Almela E, Carrasco L
Scientific reports 2017 Feb 27;7:43876
Scientific reports 2017 Feb 27;7:43876
Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
Starck SR, Jiang V, Pavon-Eternod M, Prasad S, McCarthy B, Pan T, Shastri N
Science (New York, N.Y.) 2012 Jun 29;336(6089):1719-23
Science (New York, N.Y.) 2012 Jun 29;336(6089):1719-23
Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.
Skabkin MA, Skabkina OV, Dhote V, Komar AA, Hellen CU, Pestova TV
Genes & development 2010 Aug 15;24(16):1787-801
Genes & development 2010 Aug 15;24(16):1787-801
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12840-1-AP(LGTN antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using LGTN antibody 12840-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
