PA5-76967

antibody from Invitrogen Antibodies

Targeting: IL20 IL-20, IL10D, ZCYTO10

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-76967

Provider

Invitrogen Antibodies

Product name

IL-20 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

IL-20 promotes cutaneous inflammation and peripheral itch sensation in atopic dermatitis.

Lu Z, Xiao S, Chen W, Zhu R, Yang H, Steinhoff M, Li Y, Cheng W, Yan X, Li L, Xue S, Larkin C, Zhang W, Fan Q, Wang R, Wang J, Meng J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Jun;36(6):e22334

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3 IL-20 is upregulated in MC903-treated mouse ear skin when compared to control, whereas IL-20 receptors are downregulated. In contrast, IL-20R1 transcription levels are upregulated in sensory trigeminal ganglia (TG) ipsilateral to MC903-treated ear. Representative immunofluorescence staining of IL-20 (A), and IL-20R1 plus IL-20R2 (C) in MC903- and vehicle-treated mouse ear, and their fluorescence intensity analysis (B, D); n = 5 slides/mice ( n = 3 mice). (E) Q-PCR analysis of IL-20R1 and IL-20R2. (F, G) Immunofluorescence staining of IL-20R1 in ipsi. and contra. TG to MC903-treated mice ear as depicted above (F), and IL-20R1 fluorescence intensity analysis (G); n = 5 slides/mice ( n = 3 mice). For (B, D, E and G), means +- SEMs ( n = 3); ns p > .05, * p < .05, and *** p < .001, a two-tailed Student's t -test followed by Welch's correction was used. For (A, C and F), scales = 50 um